The Standard European Vector Architecture (SEVA) is a resource for implementation of a standard for physical assembly of vector plasmids and for their non-ambiguous nomenclature.
SEVA’s plasmid backbone names take the form pSEVA_XXXX where:
- the first number denotes the antibiotic resistance marker(s) as defined in figure 1
- the second number identifies the origin of replication as defined in figure 2
- the third number identifies the cargo if any is present. Standard cargos can be found here
- the fourth number is optional and identifies the gadget if any is present.
When constructing your own pSEVA vector, replace the third digit with the name of the gene of interest you cloned in the cargo slot: pSEVA_18_eGFP.
Figure 1: SEVA ORIs
Figure 2: SEVA Markers