Start a conversation

Cloning Standards

DOULIX allows you to order either ready-to-use construct or multiple fragments to assemble your plasmid by yourself. If you choose to build it yourself, DOULIX offers you several different cloning standards. When you choose a cloning method, DOULIX will adjust accordingly and deliver you the perfect fragments to run your cloning protocol. The following guide compares several of the most popular cloning methods to help you make the decision of which is best for your specific cloning project.

  • Cloning Standards

    When it comes to moving pieces of DNA around, many methods have been developed over the years; Each one with its cons and pros. The following table compares several of the most popular cloning methods...

  • DNAmate or DNA Modular Assembling Technology

    The DNAmate is a Type IIS assembling systems, as Golden Gate or MoClo, that allows the single-pot directional assembly of up to 8 DNA fragments. Each fragment has a specific prefix and suffix that har...

  • Gibson Assembly or Isothermal Assembly Reaction

    Isothermal cloning, more commonly known as Gibson assembly, efficiently joins multiple overlapping DNA fragments in a single-tube. It takes advantage of the interplay of 3 enzymes: The 5’-exonuclease ...

  • Ligation Independent Cloning

    Ligation Independent Cloning employs the 3'-5' exonuclease activity of T4 DNA polymerase in order to create 5' overhangs on both the vector and insert. In the presence of a single free dNTP, T4 polyme...

  • Gateway® Recombination Cloning

    Gateway® cloning is a recombination based cloning method. The primary benefit of Gateway is that the reaction that moves a piece of DNA from one plasmid into another is done via a single recombination...

  • Yeast-mediated Cloning and Oligonucleotide Stitching

    Yeast-mediated cloning is very similar in principle to Gibson cloning, but instead of an in vitro reaction with purified enzymes, it takes advantage of the powerful recombination abilities of yeast. S...

  • Golden Gate or Type IIS Assembly or MoClo Assembly

    Type IIS systems, such as Golden Gate, Green Gate , and MoClo , take advantage of the unique properties of type IIS restriction endonucleases, which cut dsDNA at a specified distance from the recognit...

  • Restriction Enzyme Cloning

    Restriction enzyme (endonuclease) based molecular cloning is the classic cloning method, and for many reasons, remains one of the most popular today. Restriction enzyme are used to cut double stranded...