DOULIX’s plasmid backbone names take the form pDLX_#X#_#### where:

  • the letters pDLX are an acronym for DOULIX plasmid
  • the first number denotes the origin of replication as encoded in table 1
  • the letter X identifies the antibiotic resistance marker(s) present in the vector as encoded in table 2. Plasmid backbones with multiple resistance markers have multiple, successive letters
  • the second number identifies different versions of the same plasmid that contains point mutations that do not affect its function
  • the last four numbers after the underscore identify different versions of the same plasmid that contains different arrangement in the multiple cloning site.
NumberReplication OriginCopy NumberPurpose
1modified pMB1 derived from pUC19500-700Easy plasmid DNA purification
2F and P1 lytic derived from pSCANS-1-BNL1-2 inducible to high copyInducible copy number
3p15A derived from pMR10110-12Multi-plasmid engineered systems
4rep101, repA derived from pSC101~5Small cell to cell copy number variation
5derived from F plasmid1-2Improved plasmid stability
6pMB1 derived from pBR32215-20Multi-plasmid engineered systems
7broad host range origin by Ichiro et al.
8pSC101TS temperature sensitive origin, only propagates at 30C
9ColE1  

Table 1

CodeAntibiotic
Aampicillin
Cchloramphenicol
Eerythromycin
Ggentamycin
Kkanamycin
Nneomycin
Nanalidixic acid
Rrifampicin
Sspectinomycin
Ststreptomycin
Ttetracycline
Tmtrimethoprim
Zzeocin

Table 2

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