Cloning Standards

When it comes to moving pieces of DNA around, many methods have been developed over the years; Each one with its cons and pros. The following table compares several of the most popular cloning methods according to useful parameters:

  • Multiple fragments assembling
  • One-pot Synthesis
  • Cost per reaction
  • Excise and Replace
  • Fragment size
  • Scar-less
  • Accuracy
 Restriction Enzyme CloningGolden GateDNAMateGibson AssemblyLigation Independent CloningGateway Recombination Cloning
Multi-Assembly** * * * ** * * * ** * * * *** * *
One-pot * * * * ** * * * ** * * * *** * *
Cost** * ** * ** * *** * *
Excise/Replace* * * * ** ** * * * ** *** *
Fragment Size* * * * ** * * ** * * ** * * * ** * ** * *
Scarless* * ** * * * ** * ** * * * ** ** * *
Accuracy>80%>80%>80%>80%>80%
Available on Doulixcoming soonyesyesyesyesyes
 
 
 

LIKE THIS ARTICLE? 

Share on facebook
Share on twitter
Share on linkedin