Ligation Independent Cloning

Ligation Independent Cloning employs the 3′-5′ exonuclease activity of T4 DNA polymerase in order to create 5′ overhangs on both the vector and insert. In

Origin of replication

DOULIX’s plasmid backbone names take the form pDLX_#X#_#### where: the letters pDLX are an acronym for DOULIX plasmid the first number denotes the origin of