Matryoshka references

Author: Serena Marletta, PhD – Doulix Collection Manager One of the sweetest memories of my childhood is linked to my great grandmother’s tiramisù. Unfortunately, she

Restriction Enzyme Cloning

Restriction enzyme (endonuclease) based molecular cloning is the “classic” cloning method, and for many reasons, remains one of the most popular today. Restriction enzyme are

Gateway® Recombination Cloning

Gateway® cloning is a recombination based cloning method. The primary benefit of Gateway is that the reaction that moves a piece of DNA from one

Ligation Independent Cloning

Ligation Independent Cloning employs the 3′-5′ exonuclease activity of T4 DNA polymerase in order to create 5′ overhangs on both the vector and insert. In

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