Restriction enzyme (endonuclease) based molecular cloning is the “classic” cloning method, and for many reasons, remains one of the most popular today. Restriction enzyme are

Yeast-mediated cloning is very similar in principle to Gibson cloning, but instead of an in vitro reaction with purified enzymes, it takes advantage of the

Gateway® cloning is a recombination based cloning method. The primary benefit of Gateway is that the reaction that moves a piece of DNA from one

Ligation Independent Cloning employs the 3′-5′ exonuclease activity of T4 DNA polymerase in order to create 5′ overhangs on both the vector and insert. In

The DNAmate is a Type IIS assembling systems, as Golden Gate or MoClo, that allows the single-pot directional assembly of up to 8 DNA fragments.