Restriction Enzyme Cloning
Restriction enzyme (endonuclease) based molecular cloning is the “classic” cloning method, and for many reasons, remains one of the most popular today. Restriction enzyme are
Restriction enzyme (endonuclease) based molecular cloning is the “classic” cloning method, and for many reasons, remains one of the most popular today. Restriction enzyme are
Yeast-mediated cloning is very similar in principle to Gibson cloning, but instead of an in vitro reaction with purified enzymes, it takes advantage of the
Gateway® cloning is a recombination based cloning method. The primary benefit of Gateway is that the reaction that moves a piece of DNA from one
Ligation Independent Cloning employs the 3′-5′ exonuclease activity of T4 DNA polymerase in order to create 5′ overhangs on both the vector and insert. In
The DNAmate is a Type IIS assembling systems, as Golden Gate or MoClo, that allows the single-pot directional assembly of up to 8 DNA fragments.