Restriction Enzyme Cloning

Restriction enzyme (endonuclease) based molecular cloning is the “classic” cloning method, and for many reasons, remains one of the most popular today. Restriction enzyme are

Gateway® Recombination Cloning

Gateway® cloning is a recombination based cloning method. The primary benefit of Gateway is that the reaction that moves a piece of DNA from one

Ligation Independent Cloning

Ligation Independent Cloning employs the 3′-5′ exonuclease activity of T4 DNA polymerase in order to create 5′ overhangs on both the vector and insert. In

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