When it comes to moving pieces of DNA around, many methods have been developed over the years; Each one with its cons and pros. The following table compares several of the most popular cloning methods according to useful parameters:
- Multiple fragments assembling
- One-pot Synthesis
- Cost per reaction
- Excise and Replace
- Fragment size
- Scar-less
- Accuracy
| Restriction Enzyme Cloning | Golden Gate | DNAMate | Gibson Assembly | Ligation Independent Cloning | Gateway Recombination Cloning | |
|---|---|---|---|---|---|---|
| Multi-Assembly | * | * * * * * | * * * * * | * * * * * | * | * * * |
| One-pot | * * * * * | * * * * * | * * * * * | * | * * * | |
| Cost | * | * * * | * * * | * * * | * | * * * |
| Excise/Replace | * * * * * | * * | * * * * * | * * | * | * * |
| Fragment Size | * * * * * | * * * * | * * * * | * * * * * | * * * | * * * |
| Scarless | * * * | * * * * * | * * * | * * * * * | * * | * * * |
| Accuracy | >80% | >80% | >80% | >80% | - | >80% |
| Available on Doulix | coming soon | yes | yes | yes | yes | yes |
Doulix Team
Comments