When it comes to moving pieces of DNA around, many methods have been developed over the years; Each one with its cons and pros. The following table compares several of the most popular cloning methods according to useful parameters:
- Multiple fragments assembling
- One-pot Synthesis
- Cost per reaction
- Excise and Replace
- Fragment size
- Scar-less
- Accuracy
Restriction Enzyme Cloning | Golden Gate | DNAMate | Gibson Assembly | Ligation Independent Cloning | Gateway Recombination Cloning | |
---|---|---|---|---|---|---|
Multi-Assembly | * | * * * * * | * * * * * | * * * * * | * | * * * |
One-pot | * * * * * | * * * * * | * * * * * | * | * * * | |
Cost | * | * * * | * * * | * * * | * | * * * |
Excise/Replace | * * * * * | * * | * * * * * | * * | * | * * |
Fragment Size | * * * * * | * * * * | * * * * | * * * * * | * * * | * * * |
Scarless | * * * | * * * * * | * * * | * * * * * | * * | * * * |
Accuracy | >80% | >80% | >80% | >80% | - | >80% |
Available on Doulix | coming soon | yes | yes | yes | yes | yes |
Doulix Team
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