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Cloning Standards

When it comes to moving pieces of DNA around, many methods have been developed over the years; Each one with its cons and pros. The following table compares several of the most popular cloning methods according to useful parameters:

  • Multiple fragments assembling
  • One-pot Synthesis
  • Cost per reaction
  • Excise and Replace
  • Fragment size
  • Scar-less
  • Accuracy

Restriction Enzyme Cloning Golden Gate DNAMate Gibson Assembly Ligation Independent Cloning Gateway Recombination Cloning
Multi-Assembly * * * * * * * * * * * * * * * * * * * *
One-pot
* * * * * * * * * * * * * * * * * * *
Cost * * * * * * * * * * * * * *
Excise/Replace * * * * * * * * * * * * * * * * *
Fragment Size * * * * * * * * * * * * * * * * * * * * * * * *
Scarless * * * * * * * * * * * * * * * * * * * * *
Accuracy >80% >80% >80% >80% - >80%
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