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Origin of replication

DOULIX's plasmid backbone names take the form pDLX_#X#_#### where:

  • the letters pDLX are an acronym for DOULIX plasmid
  • the first number denotes the origin of replication as encoded in table 1
  • the letter X identifies the antibiotic resistance marker(s) present in the vector as encoded in table 2. Plasmid backbones with multiple resistance markers have multiple, successive letters
  • the second number identifies different versions of the same plasmid that contains point mutations that do not affect its function
  • the last four numbers after the underscore identify different versions of the same plasmid that contains different arrangement in the multiple cloning site.
Number Replication Origin Copy Number Purpose
1 modified pMB1 derived from pUC19 500-700 Easy plasmid DNA purification
2 F and P1 lytic derived from pSCANS-1-BNL 1-2 inducible to high copy Inducible copy number
3 p15A derived from pMR101 10-12 Multi-plasmid engineered systems
4 rep101, repA derived from pSC101 ~5 Small cell to cell copy number variation
5 derived from F plasmid 1-2 Improved plasmid stability
6 pMB1 derived from pBR322 15-20 Multi-plasmid engineered systems
7 broad host range origin
by Ichiro et al.
8 pSC101TS
temperature sensitive origin, only propagates at 30C
9 ColE1

Table 1

Code Antibiotic
A ampicillin
C chloramphenicol
E erythromycin
G gentamycin
K kanamycin
N neomycin
Na nalidixic acid
R rifampicin
S spectinomycin
St streptomycin
T tetracycline
Tm trimethoprim
Z zeocin

Table 2

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  1. Doulix Team

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